Uji Efektivitas Antibakteri Ekstrak Etanol Kulit Pisang Kepok (Musa Acuminata Balbisiana Colla) dan Daun Salam (Syzgiyum Polyanthum) Terhadap Bakteri Escherchia Coli

Noha Hanifa; Kurnia Ritma Dhanti; Kurniawan Kurniawan; Arif Mulyanto

doi:10.33627/oz.v13i2.1960

Noha Hanifa Universitas Muhammadiyah Purwokerto
Kurnia Ritma Dhanti Universitas Muhammadiyah Purwokerto
Kurniawan Kurniawan Universitas Muhammadiyah Purwokerto
Arif Mulyanto Universitas Muhammadiyah Purwokerto

DOI: https://doi.org/10.33627/oz.v13i2.1960

Keywords: Antibacterial, Banana peel, Bay leaf, Escherichia coli

Abstract

This research aims to examine the effectiveness of the combination of bay leaf extract and kepok banana peel extract as an anti Escherichia coli bacteria. This type of research is experimental with a posttest only control group design with a comparison of kepok banana peel and bay leaves, F1 (25%: 75%), F2 (50%: 50%), F3 (75%: 25%). The research results showed that the most effective extract was F1 treatment (25%:75%) with an inhibitory power of 9.7mm². The results of the statistical analysis showed that the inhibition test treatment against E. Coli bacteria using different concentrations showed a mean difference in the area of the inhibition zone with a significant value of 0.000 (p<0.05). The results of the Duncan Multiple Range Test (DMRT) showed that K+ (chloramphenicol) was significantly different from the concentrations of F1, F2 and F3. Testing the effectiveness of Kepok banana peel extract and bay leaf extract against E. coli bacteria showed that F1 treatment was effective in preventing bacterial growth against the F1 combination of 25% Kepok banana peel and 75% bay leaves.

Downloads

Download data is not yet available.

References

Alfaridz F. (2018). Review jurnal: Klasifikasi dan aktivitas farmakologi dari senyawa aktif flavonoid. Farmaka. 2018:16(3);

Ananta, I. G. B. T., Rita, W. S., & Parwata, M. O. A. (2018). Potensi Ekstrak Limbah Kulit Pisang Lokal (Musa sp) sebagai Antibakteri terhadap Escherichia coli dan Staphylococcus aureus. Cakra Kimia (Indonesian E-Journal of Applied Chemistry, 6(1), 21–29.

Apriliana, E., Ramadhian, M. R., Warganegara, E., Hasibuan, A., Studi, P., Dokter, P., Kedokteran, F., & Lampung, U. (2018). Perbandingan Daya Hambat Ekstrak Daun Jarak Pagar (Jatropha curcas Linn ) terhadap Pertumbuhan Bakteri Staphylococcus aureus dan Escherichia coli secara In Vitro Comparison of in vitro inhibitory effect of Jatropha curcas Linn extract on the growth of Staphylococcus aureus and Escherichia coli Bacteria. 5, 556–561.

Evendi Agus, 2017.Uji Fitokimia Dan Antibakteri Ekstrak Daun Salam (Syzgiyum Polyanthum) terhadap bakteri Salmonella typhi Dan Escherchia Coli Secara In Vitro.Mahakam Medical Laboratory Technology Journal.Volume II No,1 Hal 1-9, Kalimantan Timur.

Fadhilah, F., Jalani, M., Mohamad, S., Nazatul, W., & Shahidan, S. (2014). Antibacterial effects of banana pulp extracts based on different extraction methods against selected microorganisms. Asian Journal of Biomedical and Pharmaceutical Sciences, 4(36), 14–19.

Harini, K., Jerlin Showmya, J., & Geetha, N. 2014. Phytochemical Constituents Of Different Extracts From The Leaves of Chromolaena odorata (L.)

Hasniar, H. 2018. Pengaruh Variasi Konsentrasi Ekstrak Etanol Kulit Buah Pisang Kepok (Musa Paradisiaca) Dalam Bentuk Sediaan Gel Menggunakan Basisi Hpmc Terhadap Penyembuhan Luka Sayat Pada Kelinci (Oryctolagus cuniculus). Skripsi. Fakultas Kedokteran Dan Ilmu Kesehatan.

Karmilah, Reymon, Setiawan, A.M., Arifin,A.E. Musdalipah. (2019). Identifikasi Senyawa Saponin Ekstrak Etil Asetat Gonad Landak Laut (Diadema setosum L.) dan Efektivitas Antihiperkolestrol Terhadap Mencit. Journal Medika Udayana. 8(12).

Martiningsih, S. H., Kartini, P. R., & Phytokimia, S. (2023). Phytochemical Screening Test on 96% Ethanol Extract and Syzygium Water Extract. 2, 154-161.

Perintis, J., No, K., Barat, S., Sari, D. P., Rahmi, E., & Fitria, I. (2023). Fakultas Kedokteran Gigi Universitas Andalas Efektivitas Ekstrak Kulit Pisang Kepok ( Musa Paradisiaca L ) Terhadap Candida albicans dan Streptococcus mutans sebagai Pembersih Basis Akrilik Gigi Tiruan Lepasan. Andalas Dental Journal, 11(77), 35–39.

Prihatiningtyas, W., Mariani, Oramahi, Yusro, Sisillia. (2018). Uji Aktivitas Antibakteri Ekstrak Etanol Kulit Batang Mangga Kweni (Mangifera odorata Griff) terhadap Eschericha coli AATC 25922 dan Staphylococcus aureus ATCC 25923. Jurnal Tekwang. Vol 8. No 2. Hal 59-74.

Pusmarani, J., et al. Antioxidant Activity Assay of Combination of Purified Extract of Banana Peel (Musa paradisiaca Sapientum) and Andrographis Paniculata Leaves. Journal of Global Pharma technology. 2020; 12:98-104.

Rahmawati, D. P. 2018. eval_uation of Drug Use at Marsudi Waluyo Hospital, Malang Regency in 2016. Calyptra: University of Surabaya Student Scientific Journal Vol.7 No.2 (2018), 2(2), 1-12.

Sari, R., Muhani, M., & Fajriaty, I. (2017). Uji Aktivitas Antibakteri Ekstrak Etanol Daun Gaharu ( Aquilaria microcarpa Baill)Terhadap Bakteri Staphylococcus aureus dan Proteus mirabilis Antibacterial Activity of Ethanolic Leaves Extract of Agarwood ( Aquilaria microcarpa Baill ) Against Staphy. 4(3), 143–154.

Sembiring, B. M., Nasution, S., & Farmasi, F. (2021). Uji Efektivitas Ekstrak Etanol dari Kulit Pisang Kepok (Musa Paradisiaca) untuk Penyembuhan Luka Sayat Pada Tikus. 3(1), 112–121.

Sigit Purwanto, 2015. Uji Aktivitas Antibakteri Fraksi Aktif Ekstrak Daun Senggani (Melastoma malabathricum L) Terhadap Escherichia coli Jurnal Keperawatan Sriwijaya, Vol 2 no.2.

Sudirman, & Skripsa, T. H. 2020. Utilization of Traditional Medicine Services (Batra) as a Role Model for Back to Nature Medicine in the Future. ARSY: Journal of Research Applications to Society, 1(1), 45-50.

Silalahi M, 2017. Syzygium polyanthum (Wight) Walp (Botany of Secondary Metabolites and Utilization). Journal of Educational Dynamics; 10(1): 1-16.

Verdiana, M., Widarta, I.W.R. and Permana, I.D.G.M. 2018. Pengaruh Jenis Pelarut Pada Ekstraksi Menggunakan Gelombang Ultrasonik Terhadap Aktivitas Antioksidan Ekstrak Kulit Buah Lemon (Citrus limon (Linn.) Burm F.).” Jurnal Ilmu Dan Teknologi Pangan (ITEPA) 7(4):213. doi: 10.24843/itepa.2018.v07.i04.p0

Vivian, F., Apridamayanti, P., & Luliana, S. (2023). aktivitas antioksidan ekstrak air. Indonesian Journal of Pharmaceutical Education, 3(2), 235- 246.

Widyaningtias, N. M. S. R. Yustiantara, P. S., Paramita, N. L. P. . (2014). Uji Aktivitas Antibakteri Ekstrak Terpurifikasi Daun sirih hijau (Piper betleL.) Terhadap Bakteri Propionibacterium acnes.

Winiati, Rahayu, P., Nurjannah, S., & Komalasari, E. (2021). Escherichia coli: Patogenitas, Analiis, dan Kajian Risiko. Bogor: PT Penerbit IPB Press.

Wu, S. C., Liu, F., Zhu, K., & Shen, J. Z. 2019. Natural Products That Target Virulence Factors in Antibiotic-Resistant Agricultural Staphylococcus aureus. Journal of and Food Chemistry, 67 (48), 13195-13211.

Wikananda, LD. A. R. N., Hendrayana, M. A., & Pinatih, K. J. P. 2019. Efek Antibakteri Ekstrak Ethanol Kulit Batang Tanaman Cempaka Kuning (M. champaca L.) terhadap Pertumbuhan Staphylococcus aureus. Jurnal Medika, 8(5), 2597-8012.

Yana, Y. (2018). Uji Ekstrak Daun Jambu biji (Psidium guajava L.) sebagai Ovasida Keong Mas (Pomacea canaliculate L.). [Skripsi]. Fakultas Tarbiyah dan Keguruan, Universitas Islam Negeri Raden Inten.

Yanti, Y. N., & Mitika, S. 2017. Uji Efektivitas Antibakteri Ekstrak Etanol Daun Sambiloto (Andrographis paniculata Ness) terhadap Bakteri Staphylococcus aureus. Jurnal Ilmiah Ibnu Sina, 2(1),158.